ABSTRACT
Objective@#To explore the effect of tanshinone IIA on neuronal apoptosis in the compressed spinal cord and its molecular mechanism by establishing a spinal cord compression model.@*Methods@#Twenty-four SD rats weighing 250-300 g were the experimental animals. The spinal cord compression model was established by clamping the spinal cord with arterial clamp. Six rats in each group were randomly selected and randomly divided into sham group (Sham group, given intraperitoneal injection of normal saline at the same dose once a day) and spinal cord compression injury group (SCI group, given normal salts). Water intraperitoneal injection, once a day), tanshinone IIA group (TAN group, 30 mg/kg tanshinone IIA intraperitoneal injection, once a day), LY2904002 group (LY group, 0.3 mg/kg LY294002 intraperitoneal injection, 5 min after 30 mg/kg tanshinone IIA intraperitoneal injection, once 1 d). After 3 d of intervention, the motor function of rats were evaluated by inclined plane test and BBB score. The expression of apoptotic genes and PI3K/AKT signaling molecules in the compressed spinal cord were detected by qPCR, immunohistochemistry and Western blot.@*Results@#The BBB score 3.31±0.45 points, inclined angle 9.31°±1.02°, GSK-3β 0.35±0.06, CyclinD1 0.25±0.06, Bcl-2 0.38±0.06, p-PI3K 0.32±0.05, p-AKT 0.29±0.07 protein expression in SCI group were lower than those in Sham group. The protein expression of Caspase-9 3.27±0.54 and Caspase-3 2.73±0.35 in SCI group was higher than that in Sham group. The BBB score 9.31±1.02 points, inclined angle 24.95°±3.52°, GSK-3β 0.74±0.09, CyclinD1 0.69±0.11, Bcl-2 0.83±0.13, p-PI3K 0.77±0.11, p-AKT 0.69±0.08 in TAN group were higher than those in SCI group BBB score 3.31 ±0.45 points, inclined angle 9.31°±1.02°, GSK-3β 0.35±0.06, CyclinD1 0.25±0.06, Bcl-2 0.38±0.06, p-PI3K 0.32±0.05, p-AKT 0.29±0.07. The protein expression levels of Caspase-9 1.78±0.22 and Caspase-3 1.64±0.2 in TAN group were lower than those in SCI group 3.27±0.54 and Caspase-3 2.73±0.35. The BBB score, oblique angle and the expression of GSK-3β 0.43±0.07, CyclinD1 0.38±0.06, Bcl-2 0.49±0.09 in LY group were lower than those in TAN group. The protein expression of Caspase-9 2.54±0.38 and Caspase-3 2.25±0.37 in LY group were higher than TAN group.@*Conclusion@#Tanshinone IIA can inhibit the apoptosis of spinal cord tissue and alleviate the spinal cord injury in spinal cord compression rats by activating the PI3K/AKT signaling pathway.
ABSTRACT
Objective To explore the effect of tanshinone ⅡA on neuronal apoptosis in the compressed spinal cord and its molecular mechanism by establishing a spinal cord compression model.Methods Twenty-four SD rats weighing 250-300 g were the experimental animals.The spinal cord compression model was established by clamping the spinal cord with arterial clamp.Six rats in each group were randomly selected and randomly divided into sham group (Sham group,given intraperitoneal injection of normal saline at the same dose once a day) and spinal cord compression injury group (SCI group,given normal salts).Water intraperitoneal injection,once a day),tanshinone ⅡA group (TAN group,30 mg/kg tanshinone ⅡA intraperitoneal injection,once a day),LY2904002 group (LY group,0.3 mg/kg LY294002 intraperitoneal injection,5 min after 30 mg/kg tanshinone ⅡA intraperitoneal injection,once 1 d).After 3 d of intervention,the motor function of rats were evaluated by inclined plane test and BBB score.The expression of apoptotic genes and PI3K/AKT signaling molecules in the compressed spinal cord were detected by qPCR,immunohistochemistry and Western blot.Results The BBB score 3.31±0.45 points,inclined angle 9.31°±1.02°,GSK-3β 0.35±0.06,CyclinD1 0.25±0.06,Bcl-2 0.38±0.06,p-PI3K 0.32±0.05,p-AKT 0.29±0.07 protein expression in SCI group were lower than those in Sham group.The protein expression of Caspase-9 3.27±0.54 and Caspase-3 2.73±0.35 in SCI group was higher than that in Sham group.The BBB score 9.31±1.02 points,inclined angle 24.95°±3.52°,GSK-3β 0.74±0.09,CyclinD1 0.69±0.11,Bcl-2 0.83±0.13,p-PI3K 0.77±0.11,p-AKT 0.69±0.08 in TAN group were higher than those in SCI group BBB score 3.31 ±0.45 points,inclined angle 9.31°±1.02°,GSK-3β 0.35±0.06,CyclinD1 0.25±0.06,Bcl-2 0.38±0.06,p-PI3K 0.32±0.05,p-AKT 0.29± 0.07.The protein expression levels of Caspase-9 1.78±0.22 and Caspase-3 1.64±0.2 in TAN group were lower than those in SCI group 3.27±0.54 and Caspase-3 2.73±0.35.The BBB score,oblique angle and the expression of GSK-3β 0.43±0.07,CyclinD1 0.38±0.06,Bcl-2 0.49±0.09 in LY group were lower than those in TAN group.The protein expression of Caspase-9 2.54±0.38 and Caspase-3 2.25±0.37 in LY group were higher than TAN group.Conclusion Tanshinone ⅡA can inhibit the apoptosis of spinal cord tissue and alleviate the spinal cord injury in spinal cord compression rats by activating the PI3K/AKT signaling pathway.